AMP and IMP Dissociate Actomyosin into Actin and Myosin | Zendy

Akihiro Okitani | Zendy; Naoki Ichinose | Zendy; Miki KOZA | Zendy; Kensuke Yamanaka | Zendy; Koshiro Migita | Zendy; Masanori MATSUISHI | Zendy

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AMP and IMP Dissociate Actomyosin into Actin and Myosin

Author(s) -

Akihiro Okitani,

Naoki Ichinose,

Miki KOZA,

Kensuke Yamanaka,

Koshiro Migita,

Masanori MATSUISHI

Publication year - 2008

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.80128

Subject(s) - myosin , actin , chemistry , biophysics , microbiology and biotechnology , biochemistry , biology

We investigated to determine why heating of squid muscle at 60 degrees C induced the liberation of actin from myofibrils. When a mixture of a myofibrillar fraction and a low-molecular sarcoplasmic fraction prepared from squid muscle was heated at 60 degrees C, actin liberation occurred. When a myofibrillar fraction was heated with ATP, AMP, or IMP, actin liberation occurred. Hence, AMP is perhaps one of the factors causing actin liberation in postmortem squid muscle. It was found that AMP and IMP reversibly dissociated actomyosin of chicken, bovine, and porcine skeletal muscles into actin and myosin on incubation at 0 degrees C at pH 7.2 in 0.2 M KCl. These results led us to conclude that AMP and IMP were the most responsible factors causing actin liberation from myofibrils in the heated muscle and causing reversible dissociation of actomyosin on storage of skeletal muscle at a low temperature. Hence, AMP and IMP are possible factors causing the resolution of rigor mortis in muscles.

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