Determination of the Luciferin Contents in Luminous and Non-Luminous Beetles | Zendy

Yuichi Oba | Zendy; Takeru Shintani | Zendy; Takanori Nakamura | Zendy; Makoto Ojika | Zendy; S Inouye | Zendy

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Determination of the Luciferin Contents in Luminous and Non-Luminous Beetles

Author(s) -

Yuichi Oba,

Takeru Shintani,

Takanori Nakamura,

Makoto Ojika,

S Inouye

Publication year - 2008

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.80017

Subject(s) - luciferin , firefly protocol , luminous efficacy , bioluminescence , luminous intensity , fluorescence , luminescence , chemistry , luciferase , biology , biochemistry , optics , physics , zoology , organic chemistry , layer (electronics) , gene , transfection

The contents of firefly luciferin in luminous and non-luminous beetles were determined by the methods of HPLC with fluorescence detection and the luminescence reaction of luciferin and firefly luciferase. Luminous cantharoids and elaterids contained various amounts of luciferin in the range of pmol to hundreds of nmol, but no luciferin was detected in the non-luminous cantharoids and elaterids.

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