Production of Poly(3-hydroxybutyrate) from Waste Potato Starch
Author(s) -
Richard Haas,
Bo Jin,
Florian Zepf
Publication year - 2008
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.70503
Subject(s) - starch , maltose , ralstonia , food science , potato starch , chemistry , biomass (ecology) , polyhydroxyalkanoates , poly 3 hydroxybutyrate , carbon source , carbon fibers , bioplastic , nuclear chemistry , pulp and paper industry , biochemistry , waste management , materials science , bacteria , agronomy , enzyme , biology , sucrose , genetics , composite number , composite material , engineering
There has been a considerable interest in using low cost carbon substrates for the production of Poly(3-hydroxybutyrate) (PHB). We have shown that saccharified waste potato starch can be used as a viable alternative carbon source in high cell density PHB production. Using Ralstonia eutropha NCIMB 11599 with phosphate limitation, 179 g/l biomass, 94 g/l PHB, Y(biomass/starch) = 0.46 g/g, Y(PHB/starch) = 0.22 g/g, and PHB productivity = 1.47 g/(l*h) were achieved. Residual maltose accumulated in the fed-batch reactor but caused no noticeable inhibition. Performance with saccharified starch was virtually identical to that with glucose.
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