Biocalorimetric Studies of the Metabolic Activity ofPseudomonas aeruginosaAerobically Grown in a Glucose-Limited Complex Growth Medium | Zendy

Senthilkumar Sivaprakasam | Zendy; Surianarayanan Mahadevan | Zendy; Susheela Rajakumar | Zendy

Open Access

Biocalorimetric Studies of the Metabolic Activity ofPseudomonas aeruginosaAerobically Grown in a Glucose-Limited Complex Growth Medium

Author(s) -

Senthilkumar Sivaprakasam,

Surianarayanan Mahadevan,

Susheela Rajakumar

Publication year - 2008

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.70476

Subject(s) - pseudomonas aeruginosa , chemistry , growth rate , substrate (aquarium) , bacterial growth , calorimetry , mesophile , food science , calorimeter (particle physics) , aeration , yield (engineering) , strain (injury) , isothermal microcalorimetry , metabolic flux analysis , bacteria , biochemistry , metabolism , biology , thermodynamics , enthalpy , organic chemistry , ecology , genetics , geometry , mathematics , physics , anatomy , detector , electrical engineering , engineering

Biocalorimetric experiments were performed to investigate the aerobic growth of Pseudomonas aeruginosa, isolated from tannery saline wastewater. Growth factors (pH, Inoculum size, carbon source, temperature, aeration rate, and agitation rate) were optimized in shaker and calorimeter based on the growth of P. aeruginosa and heat generation rates. A limiting value of 0.2% glucose concentration was found to be optimum for the growth of P. aeruginosa in a complex growth medium, and the heat flux (q(r)) profiles resulting from the metabolic activity of P. aeruginosa further confirmed this observation. The bacterial growth profile was found to correlate well with the metabolic heat generated. Heat-yield values were calculated for both glucose consumption and the growth of P. aeruginosa from the calorimetric results. Metabolic shifts in substrate uptake from glucose to peptone present in growth medium was observed by the variations in heat-flux profile. The calorimetric data presented in this study should be useful in understanding the behavior of the isolated bacterial strain in degrading complex and mixed substrates commonly observed in tannery saline waste stream, and further to extend the results for scale-up studies.

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