Open AccessMulti-Well Chip for Forming a Uniform Embryoid Body in a Tiny Droplet with Mouse Embryonic Stem Cells
Author(s) -
C. S. Kim,
In Hye Lee,
Kangsun Lee,
Sung-Shin Ryu,
Sang Ho Lee,
Kyu-Jung Lee,
Jinwoo Lee,
Ji Yoon Kang,
Tae Song Kim
Publication year - 2007
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.70384
Subject(s) - embryoid body , embryonic stem cell , radius of curvature , drop (telecommunication) , suspension culture , spheroid , materials science , curvature , chemistry , biophysics , nanotechnology , biology , anatomy , cell culture , adult stem cell , mean curvature , mathematics , geometry , engineering , biochemistry , mean curvature flow , electrical engineering , genetics , gene
A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 x 10(3) to 20 x 10(3). The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells.
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