Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath ofLeptothrix cholodnii | Zendy

Hiroko Makita | Zendy; Yuichi Nakahara | Zendy; H. Fukui | Zendy; Yohei Miyanoiri | Zendy; Masato Katahira | Zendy; Hiroko Seki | Zendy; Minoru Takeda | Zendy; Junichi Koizumi | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath ofLeptothrix cholodnii

Author(s) -

Hiroko Makita,

Yuichi Nakahara,

H. Fukui,

Yohei Miyanoiri,

Masato Katahira,

Hiroko Seki,

Minoru Takeda,

Junichi Koizumi

Publication year - 2006

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.70.1265

Subject(s) - residue (chemistry) , cysteine , chemistry , glycoconjugate , reagent , mass spectrometry , peptide , polysaccharide , chromatography , biochemistry , organic chemistry , enzyme

The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research