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Cloning and Characterization of an RNase-Related Protein Gene Preferentially Expressed in Rice Stems
Author(s) -
Junya Wei,
Anming Li,
Li Yin,
Jing Wang,
Xiaobin Liu,
Liu Liangshi,
Zeng-Fu Xu
Publication year - 2006
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.70.1041
Subject(s) - gene , open reading frame , genetics , rnase p , biology , oryza sativa , mutant , intron , cloning (programming) , amino acid , microbiology and biotechnology , peptide sequence , rna , computer science , programming language
RNase-related proteins (RRPs) are S- and S-like RNase homologs lacking the active site required for RNase activity. Here we describe the cloning and characterization of the rice (Oryza sativa) RRP gene (OsRRP). A single copy of OsRRP occurs in the rice genome. OsRRP contains three introns and an open reading frame encoding 252 amino acids, with the replacement of two histidines involved in the active site of RNase by lysine and tyrosine respectively. OsRRP is preferentially expressed in stems of wild-type rice and is significantly down-regulated in an increased tillering dwarf mutant ext37.

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