Production of Heterogeneous Dimer Lignostilbenedioxygenase II fromlsdAandlsdBinEscherichia coliCells | Zendy

Shigehiro Kamoda | Zendy; Tamami Terada | Zendy; Yoshimasa Saburi | Zendy

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Production of Heterogeneous Dimer Lignostilbenedioxygenase II fromlsdAandlsdBinEscherichia coliCells

Author(s) -

Shigehiro Kamoda,

Tamami Terada,

Yoshimasa Saburi

Publication year - 2005

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.69.635

Subject(s) - isozyme , escherichia coli , dimer , ion chromatography , biochemistry , chemistry , escherichia , enzyme , gene , microbiology and biotechnology , biology , organic chemistry

The lignostilbenedioxygenase isozyme I and III genes, lsdA and lsdB, were coexpressed within the same Escherichia coli cells. The lignostilbenedioxygenase isozymes produced were separated on QAE anion-exchange column chromatography. Three parts of active fractions corresponding to alphaalpha, alphabeta, and betabeta dimers were detected. The purified isozyme from second active fractions corresponded to the native heterogeneous isozyme II.

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