Effects of Serum Deprivation on Expression of Proteolytic-Related Genes in Chick Myotube Cultures | Zendy

Kazuki Nakashima | Zendy; Makoto Yamazaki | Zendy; Hiroyuki Abé | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Effects of Serum Deprivation on Expression of Proteolytic-Related Genes in Chick Myotube Cultures

Author(s) -

Kazuki Nakashima,

Makoto Yamazaki,

Hiroyuki Abé

Publication year - 2005

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.69.623

Subject(s) - calpain , myogenesis , proteolysis , cathepsin , cathepsin b , ubiquitin , cathepsin d , biology , proteasome , microbiology and biotechnology , myofibril , cathepsin l , gene expression , complementary dna , gene , biochemistry , enzyme

We previously reported that serum deprivation stimulates myofibrillar proteolysis in chick myotubes. In the present study, we examined the effect of serum deprivation on expression of the proteolytic-related genes (ubiquitin, proteasome, calpains, and cathepsin B) by real-time PCR of cDNA in chick myotubes. Myotubes were incubated with serum-free medium for 24 h. Ubiquitin and proteasome subunits (C1 and C2) and calpains (m-, mu-, and p94/calpain-3) but not cathepsin B mRNA expression were increased by serum deprivation. These results indicate that serum deprivation stimulates ubiquitin-proteasome and calpain proteolytic pathways, resulting in an increase in myofibrillar proteolysis in chick myotubes.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research