Open AccessEffect of Culture Conditions on Production of 5-Aminolevulinic Acid by RecombinantEscherichia coli
Author(s) -
DaeHee Lee,
Woojin Jun,
Dong-Hoon Shin,
HongYon Cho,
Bum-Shik Hong
Publication year - 2005
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.69.470
Subject(s) - dehydratase , porphobilinogen synthase , escherichia coli , industrial fermentation , biochemistry , chemistry , porphobilinogen , enzyme , fermentation , recombinant dna , nitrogen , overproduction , atp synthase , gene , organic chemistry
Aminolevulinic acid (ALA) is formed by the enzyme ALA synthase (hemA gene). Then ALA is converted to Porphobilinogen (PBG) by the ALA dehydratase (hemB gene). For the overproduction of ALA, we used an Escherichia coli BL21(DE3) containing a hemA gene from Bradyrhzobium japonicum, which was created in our previous work. The effects of pH on the ALA synthase and ALA dehydratase were investigated. The ALA synthase and ALA dehydratase activities were dependent on the pH of the medium, with maximal activities occurring at pH 6.5 and 8.0 respectively. At pH 6.5, extracellular ALA reached 23 mM in a jar-fermenter. In addition, the effects of some nutritional factors, such as nitrogen source and the ratio of carbon to nitrogen (C/N) on the fermentative production of ALA were investigated. The highest ALA production was found with 8:1 of C/N ratio. Among various nitrogen sources, the tryptone might be a useful one for ALA production.
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