Structural and Functional Analysis of pTB6 from Bifidobacterium longum | Zendy

Kunihito Tanaka | Zendy; Kazuya SAMURA | Zendy; Yasunobu Kano | Zendy

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Structural and Functional Analysis of pTB6 from Bifidobacterium longum

Author(s) -

Kunihito Tanaka,

Kazuya SAMURA,

Yasunobu Kano

Publication year - 2005

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.69.422

Subject(s) - bifidobacterium longum , plasmid , shuttle vector , rolling circle replication , biology , genetics , nucleotide , sequence (biology) , homologous chromosome , replicon , nucleic acid sequence , dna , bifidobacterium , vector (molecular biology) , recombinant dna , microbiology and biotechnology , dna replication , bacteria , gene , lactobacillus

The complete nucleotide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB aa sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44, suggesting that pTB6 replicates by the rolling circle mechanism. The putative MembB, MobA, and protein encoding from orf (Orf) I detected were nonessential for plasmid replication. We constructed an immobile shuttle vector from pTB6 and pUC18, which transformed B. longum with a high efficiency of 2.5 x 10(6) transformants/microg DNA.

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