Cold-Active Polygalacturonase from Psychrophilic-Basidiomycetous Yeast Cystofilobasidium capitatum Strain PPY-1 | Zendy

Tomoyuki Nakagawa | Zendy; Toshinori Nagaoka | Zendy; Tatsuro Miyaji | Zendy; Noboru Tomizuka | Zendy

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Cold-Active Polygalacturonase from Psychrophilic-Basidiomycetous Yeast Cystofilobasidium capitatum Strain PPY-1

Author(s) -

Tomoyuki Nakagawa,

Toshinori Nagaoka,

Tatsuro Miyaji,

Noboru Tomizuka

Publication year - 2005

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.69.419

Subject(s) - psychrophile , pectinase , strain (injury) , pectinesterase , pectin , chemistry , yeast , molecular mass , substrate (aquarium) , polysaccharide , enzyme , cleavage (geology) , food science , biochemistry , biology , ecology , paleontology , fracture (geology) , anatomy

We purified and characterized a cold-active polygalacturonase (PG) from the extracellular fraction of Cystofilobasidium capitatum strain PPY-1. The purified PG from strain PPY-1 has a molecular mass of about 44 kDa, and exhibited high activity at 0 degrees C, although its optimum temperature was 45 degrees C. Although the Km value for polygalacturonate as a substrate at 45 degrees C was found to be 11.2 mg/ml, it decreased gradually with decreasing temperature, and it was 0.66 mg/ml at 0 degrees C. Moreover, its cleavage pattern was of the endo-type. These findings might indicate that PG from strain PPY-1 is a novel type of cold-active endo-PG that is able to degrade pectin compounds at low temperatures.

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