Transient RNAi Induction against Endogenous Genes in Arabidopsis Protoplasts Using in Vitro-Prepared Double-Stranded RNA | Zendy

Chung-Il An | Zendy; Aki SAWADA | Zendy; Yoshiaki Kawaguchi | Zendy; Eiichiro Fukusaki | Zendy; Akio Kobayashi | Zendy

Open Access

Transient RNAi Induction against Endogenous Genes in Arabidopsis Protoplasts Using in Vitro-Prepared Double-Stranded RNA

Author(s) -

Chung-Il An,

Aki SAWADA,

Yoshiaki Kawaguchi,

Eiichiro Fukusaki,

Akio Kobayashi

Publication year - 2005

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.69.415

Subject(s) - arabidopsis , rna interference , gene , gene silencing , biology , rna , endogeny , transgene , rna silencing , protoplast , microbiology and biotechnology , arabidopsis thaliana , gene expression , genetics , in vitro , computational biology , mutant , biochemistry

RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double-stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. Biochem., 67, 2674-2677 (2003)]. This simple system should be useful for functional analysis of genes involved in fundamental cellular processes.

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