Analysis of Chitin at the Hyphal Tip ofCandida albicansUsing Calcofluor White | Zendy

Hiroshi Watanabe | Zendy; Masayuki Azuma | Zendy; Koichi Igarashi | Zendy; Hiroshi Ooshima | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Analysis of Chitin at the Hyphal Tip ofCandida albicansUsing Calcofluor White

Author(s) -

Hiroshi Watanabe,

Masayuki Azuma,

Koichi Igarashi,

Hiroshi Ooshima

Publication year - 2005

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.69.1798

Subject(s) - chitin , candida albicans , hypha , cell wall , staining , chemistry , microbiology and biotechnology , corpus albicans , fluorescence , sugar , biochemistry , biology , chitosan , optics , genetics , physics

Staining with calcofluor white (CFW), which is known to bind chitin-rich areas of the cell wall, revealed a difference in the fluorescence intensity at the hyphal tip between Candida albicans hyphal cells that were grown in modified Lee (M-Lee) and SPG media. The fluorescence intensity at the tip increased with the addition of salts and sugar to SPG. The chitin levels per dry cell weight in cells grown in modified Lee and SPG with 1.0 M NaCl were also higher than in SPG. These results suggest that chitin synthesis at the tip of C. albicans might be activated by the addition of salts and sugar to a medium.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research