Culture-Independent Analysis of Fecal Microbiota in Cattle | Zendy

Yuhei Ozutsumi | Zendy; Hidenori Hayashi | Zendy; Mitsuo Sakamoto | Zendy; Hisao ITABASHI | Zendy; Yoshimi Benno | Zendy

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Culture-Independent Analysis of Fecal Microbiota in Cattle

Author(s) -

Yuhei Ozutsumi,

Hidenori Hayashi,

Mitsuo Sakamoto,

Hisao ITABASHI,

Yoshimi Benno

Publication year - 2005

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.69.1793

Subject(s) - firmicutes , actinobacteria , bacteroidetes , proteobacteria , biology , feces , bacterial phyla , phylogenetic tree , 16s ribosomal rna , microbiology and biotechnology , library , hindgut , bacteria , genetics , gene , ecology , larva , midgut

The phylogenetic diversity of the fecal bacterial community in Holstein cattle was determined by 16S ribosomal RNA gene sequence analysis. The sequences were affiliated with the following phyla: Firmicutes (81.3%), Bacteroidetes (14.4%), Actinobacteria (2.5%), and Proteobacteria (1.4%). The Clostridium leptum subgroup was the most phylogenetically diverse group in cattle feces. In addition, a number of previously uncharacterized and unidentified bacteria were recognized in clone libraries.

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