An Inhibitor of Glucosylceramide Synthase Inhibits the Human Enzyme, but Not Enzymes from Other Organisms | Zendy

Inga Hillig | Zendy; Dirk Warnecke | Zendy; Ernst Heinz | Zendy

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An Inhibitor of Glucosylceramide Synthase Inhibits the Human Enzyme, but Not Enzymes from Other Organisms

Author(s) -

Inga Hillig,

Dirk Warnecke,

Ernst Heinz

Publication year - 2005

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.69.1782

Subject(s) - enzyme , biochemistry , yeast , biosynthesis , atp synthase , biology , bacteria , chemistry , genetics

Specific inhibitors of glucosylceramide biosynthesis are used as drugs for the treatment of some human diseases correlated to glycosphingolipid metabolism. The target of the presently available inhibitors is the human glucosylceramide synthase (GCS), but effects on enzymes from other organisms have not been studied. We expressed cDNAs encoding GCS enzymes from lower animals, plants, fungi, and bacteria in the yeast P. pastoris. In vitro GCS assays with the GCS inhibitor D-threo-1-(3',4'-ethylenedioxy)phenyl-2-palmitoylamino-3-pyrrolidino-1-propanol showed that this inhibitor did not affect non-human GCS enzymes.

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