Improved Expression of Novel Red- and Green-emitting Luciferases ofPhrixothrixRailroad Worms in Mammalian Cells
Author(s) -
Yoshihiro Nakajima,
Takuma Kimura,
Chie Suzuki,
Yoshihiro Ohmiya
Publication year - 2004
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.68.948
Subject(s) - luciferases , reporter gene , gene , gene expression , biology , chemistry , microbiology and biotechnology , biochemistry , transfection , luciferase
Luciferases are widely used for the quantitative monitoring of gene expression in a variety of organisms. We successfully expressed novel red- and green-emitting luciferases of Phrixothrix railroad worms in mammalian cells in combination with the Kozak sequence and the CAG promoter. The characteristic properties of these luciferases indicate that they are appropriate reporter genes for the simultaneous monitoring of two gene expressions.
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