Transient Assay System for the Analysis ofPR-1aGene Promoter in Tobacco BY-2 Cells | Zendy

Sachiko Ono | Zendy; Tsuneyuki Tanaka | Zendy; Yuriko Watakabe | Zendy; Kazuyuki Hiratsuka | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Transient Assay System for the Analysis ofPR-1aGene Promoter in Tobacco BY-2 Cells

Author(s) -

Sachiko Ono,

Tsuneyuki Tanaka,

Yuriko Watakabe,

Kazuyuki Hiratsuka

Publication year - 2004

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.68.803

Subject(s) - luciferase , reporter gene , gene expression , gene , npr1 , salicylic acid , microbiology and biotechnology , biology , promoter , regulation of gene expression , systemic acquired resistance , arabidopsis , chemistry , transfection , genetics , mutant , medicine , heart failure , natriuretic peptide

In order to develop a rapid and versatile assay system suitable for the analysis of regulated expression of tobacco pathogenesis-related protein 1a (PR-1a) gene, we investigated the use of the transient gene expression system in tobacco BY-2 cells by microprojectile bombardment. Using dual luciferase assay as a reporter gene expression detection system, we observed significant induction of PR-1a promoter activity by salicylic acid (SA) treatment. On the other hand, treatment with 4-hydroxybenzoic acid (4-HBA) resulted in no detectable increase in luciferase activity. Co-expression of a trans-acting factor, the NPR1/NIM1 protein of Arabidopsis, resulted in the induction of higher expression levels of the PR-1a promoter. These results suggest that the assay system is applicable for the analysis of factors involved in the regulated expression of SA-inducible defense-related genes.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research