Genetic Analysis of an IncompletebioOperon in a Biotin Auxotrophic Strain ofBacillus subtilisNatto OK2 | Zendy

Mayumi Sasaki | Zendy; Fujio Kawamura | Zendy; Yasurou Kurusu | Zendy

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Genetic Analysis of an IncompletebioOperon in a Biotin Auxotrophic Strain ofBacillus subtilisNatto OK2

Author(s) -

Mayumi Sasaki,

Fujio Kawamura,

Yasurou Kurusu

Publication year - 2004

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.68.739

Subject(s) - operon , bacillus subtilis , complementation , auxotrophy , strain (injury) , mutant , biology , escherichia coli , l arabinose operon , microbiology and biotechnology , gene , biotin , genetic analysis , genetics , bacteria , anatomy

We describe the genetic analysis of the bio operon of the biotin auxotrophic Bacillus subtilis natto OK2 strain. The OK2 strain would only cross-feed with the Escherichia coli bioB mutant and also grew well in medium containing dethiobiotin. Sequencing analysis revealed two significant genetic alterations in the bioW and bioF genes within the bio operon of the OK2 strain. Complementation analysis with B. subtilis 168 bio mutants demonstrated that only the bioB gene could complement, but other bio operon genes could not. A bio(+) transformant, isolated from an OK2 strain, has biotin autotrophy.

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