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Antioxidative Activity of a Zinc-Chelating Substance in Coffee
Author(s) -
Wen Xu,
Makiko Takenaka,
Masatsune Murata,
Seiichi Homma
Publication year - 2004
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.68.2313
Subject(s) - chemistry , zinc , scavenging , abts , chelation , antioxidant , linoleic acid , caffeic acid , nuclear chemistry , organic chemistry , food science , dpph , fatty acid
Coffee brew contains a brownish zinc-chelating polymer designated ApV. ApV was prepared from the precipitate formed in a solution of instant coffee by adding ZnCl(2) and purified using ion-exchange and cellulose column chromatographies. The antioxidative activities of ApV and related compounds were evaluated in this study. The free-radical scavenging activity of ApV estimated by ABTS assay was at a similar level to that of instant coffee, while the O(2)(-) scavenging activity of ApV, which is superoxide dismutase-like activity, was lower than that of instant coffee. The hydroxyl-radical scavenging activity of ApV was higher than that of instant coffee, and the auto-oxidation of linoleic acid was more strongly inhibited by ApV than by caffeic acid.

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