Substrate-dependent Activation of Thermolysin by Salt | Zendy

H. Oneda | Zendy; Yuko Muta | Zendy; Kuniyo Inouye | Zendy

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Substrate-dependent Activation of Thermolysin by Salt

Author(s) -

H. Oneda,

Yuko Muta,

Kuniyo Inouye

Publication year - 2004

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.68.1811

Subject(s) - thermolysin , salt (chemistry) , substrate (aquarium) , chemistry , materials science , biochemistry , biology , enzyme , organic chemistry , ecology , trypsin

Salt-activation of thermolysin was examined using a positively charged fluorescent substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N(3)-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH(2) [MOCAc-PLGL(Dpa)AR]. Thermolysin activity increased in a biphasic exponential fashion and was 40 times higher in the presence of 4 M NaCl than in its absence. The degree of activation at X M NaCl was expressed as 4.7(x) when [NaCl](o) < 0.5 M and 2.3(x) when [NaCl](o) > 0.5 M respectively.

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