Cloning and Expression Analysis of a MAPKKK Gene and a Novel Nodulin Gene ofLotus japonicus | Zendy

Natsuko Kinoshita | Zendy; Yasuhiro Ooki | Zendy; Yuichi Deguchi | Zendy; Svetlana A. Chechetka | Zendy; Hiroshi Kouchi | Zendy; Yosuke Umehara | Zendy; Katsura Izui | Zendy; Shingo Hata | Zendy

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Cloning and Expression Analysis of a MAPKKK Gene and a Novel Nodulin Gene ofLotus japonicus

Author(s) -

Natsuko Kinoshita,

Yasuhiro Ooki,

Yuichi Deguchi,

Svetlana A. Chechetka,

Hiroshi Kouchi,

Yosuke Umehara,

Katsura Izui,

Shingo Hata

Publication year - 2004

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.68.1805

Subject(s) - lotus japonicus , lotus , protein kinase a , map kinase kinase kinase , biology , map2k7 , protein kinase r , map3k7 , cyclin dependent kinase 2 , c raf , mitogen activated protein kinase kinase , gene , complementary dna , microbiology and biotechnology , kinase , genetics , botany , mutant

We isolated a cDNA encoding mitogen-activated protein kinase kinase kinase alpha, designated LjM3Kalpha, from Lotus japonicus, a model legume. The gene was expressed constitutively in roots, root nodules, and shoots. We also identified a novel nodulin gene, LjNUF, that shows specific expression in nodules. LjNUF resembles the C-terminal half of a hypothetical protein (pir//D85436), the N-terminal half of which is similar to a portion of mitogen-activated protein kinase kinase kinase gamma. Although LjNUF was predicted to be a secreted protein, its function remains to be clarified.

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