The Natural Intron Sequence of Human Tyrosine Pre-transfer RNA Is Not a Temporal Stabilizer for Cloverleaf Structure | Zendy

Tomoaki Ando | Zendy; Terumichi Tanaka | Zendy; Yo Kikuchi | Zendy

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The Natural Intron Sequence of Human Tyrosine Pre-transfer RNA Is Not a Temporal Stabilizer for Cloverleaf Structure

Author(s) -

Tomoaki Ando,

Terumichi Tanaka,

Yo Kikuchi

Publication year - 2004

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.68.1782

Subject(s) - intron , transfer rna , sequence (biology) , stabilizer (aeronautics) , rna , biology , tyrosine , genetics , computational biology , biochemistry , gene , mechanical engineering , engineering

We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pre-transfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it. Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.

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