Direct Evidence of Interaction of a Green Tea Polyphenol, Epigallocatechin Gallate, with Lipid Bilayers by Solid-state Nuclear Magnetic Resonance | Zendy

Shigenori Kumazawa | Zendy; Katsuko Kajiya | Zendy; Akira Naito | Zendy; Hazime Saitô | Zendy; Satoru Tuzi | Zendy; Michikazu Tanio | Zendy; Masayuki Suzuki | Zendy; Fumio Nanjo | Zendy; Eri Suzuki | Zendy; Tsutomu Nakayama | Zendy

Open Access

Direct Evidence of Interaction of a Green Tea Polyphenol, Epigallocatechin Gallate, with Lipid Bilayers by Solid-state Nuclear Magnetic Resonance

Author(s) -

Shigenori Kumazawa,

Katsuko Kajiya,

Akira Naito,

Hazime Saitô,

Satoru Tuzi,

Michikazu Tanio,

Masayuki Suzuki,

Fumio Nanjo,

Eri Suzuki,

Tsutomu Nakayama

Publication year - 2004

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.68.1743

Subject(s) - epigallocatechin gallate , polyphenol , solid state nuclear magnetic resonance , gallate , epicatechin gallate , green tea , lipid bilayer , chemistry , nuclear magnetic resonance , food science , biochemistry , physics , antioxidant , nuclear chemistry , membrane

The interaction of a tea catechin, epigallocatechin gallate (EGCg), with the model membrane of dimyristoylphosphatidylcholine (DMPC) was studied by solid-state (31)P and (2)H NMR. The (31)P chemical shift anisotropy of the DMPC phosphate group decreased on addition of EGCg. The (2)H NMR spectrum of [4-(2)H]EGCg, which is deuterated at the 4-position, in the DMPC liposomes gave deuterium nuclei with much smaller quadrupole splittings than those in the solid phase. These (31)P and (2)H NMR observations provide direct experimental evidence that the EGCg molecule interacts with the lipid bilayers.

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