Proteomic Identification of α-Amylase Isoforms Encoded byRAmy3B/3Cfrom Germinating Rice Seeds | Zendy

Yohei Nanjo | Zendy; Satoru Asatsuma | Zendy; K. Itoh | Zendy; Hidetaka Hori | Zendy; Toshiaki Mitsui | Zendy

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Proteomic Identification of α-Amylase Isoforms Encoded byRAmy3B/3Cfrom Germinating Rice Seeds

Author(s) -

Yohei Nanjo,

Satoru Asatsuma,

K. Itoh,

Hidetaka Hori,

Toshiaki Mitsui

Publication year - 2004

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.68.112

Subject(s) - gene isoform , amylase , oryza sativa , biology , biochemistry , enzyme , microbiology and biotechnology , peptide mass fingerprinting , affinity chromatography , polyacrylamide gel electrophoresis , gene , chemistry , proteomics

We isolated and identified 10 alpha-amylase isoforms by using beta-cyclodextrin Sepharose affinity column chromatography and two-dimensional polyacrylamide gel electrophoresis from germinating rice (Oryza sativa L.) seeds. Immunoblots with anti-alpha-amylase I-1 and II-4 antibodies indicated that 8 isoforms in 10 are distinguishable from alpha-amylase I-1 and II-4. Peptide mass fingerprinting analysis showed that there exist novel isoforms encoded by RAmy3B and RAmy3C genes. The optimum temperature for enzyme reaction of the RAmy3B and RAmy3C coding isoforms resembled that of alpha-amylase isoform II-4 (RAmy3D). Furthermore, complex protein polymorphism resulted from a single alpha-amylase gene was found to occur not only in RAmy3D, but also in RAmy3B.

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