Microbial Production of 2-Deoxyribose 5-Phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2′-Deoxyribonucleosides | Zendy

Jun Ogawa | Zendy; Kyota Saito | Zendy; Takafumi Sakai | Zendy; Nobuyuki Horinouchi | Zendy; Takako Kawano | Zendy; Seiichiro Matsumoto | Zendy; Mie Sasaki | Zendy; Yoichi Mikami | Zendy; Sakayu Shimizu | Zendy

Open Access

Microbial Production of 2-Deoxyribose 5-Phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2′-Deoxyribonucleosides

Author(s) -

Jun Ogawa,

Kyota Saito,

Takafumi Sakai,

Nobuyuki Horinouchi,

Takako Kawano,

Seiichiro Matsumoto,

Mie Sasaki,

Yoichi Mikami,

Sakayu Shimizu

Publication year - 2003

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.67.933

Subject(s) - dihydroxyacetone phosphate , deoxyribose , deoxyribonucleosides , triosephosphate isomerase , chemistry , acetaldehyde , phosphate , glyceraldehyde , purine nucleoside phosphorylase , deoxyribonucleoside , methylglyoxal , biochemistry , organic chemistry , stereochemistry , nucleic acid , enzyme , purine , ethanol , dehydrogenase

2-Deoxyribose 5-phosphate was produced from acetaldehyde and dihydroxyacetone phosphate via D-glyceraldehyde 3-phosphate by Klebsiella pneumoniae B-4-4 through deoxyriboaldolase- and triosephosphate isomerase-catalyzing reactions. Under the optimum conditions, 98.7 mM 2-deoxyribose 5-phosphate was produced from 200 mM acetaldehyde and 117 mM dihydroxyacetone phosphate in 2 h with a molar yield of 84%. The 2-deoxyriobse 5-phosphate produced was directly transformed to 2'-deoxyribonucleoside by phosphopentomutase- and nucleoside phosphorylase-catalyzing reactions.

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