Targeted Gene Disruption of the Neuronal Calcium Sensor 1 Homologue in Rice Blast Fungus,Magnaporthe grisea | Zendy

K Saitoh | Zendy; Tsutomu Arie | Zendy; Tohru Teraoka | Zendy; Isamu Yamaguchi | Zendy; Takashi Kamakura | Zendy

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Targeted Gene Disruption of the Neuronal Calcium Sensor 1 Homologue in Rice Blast Fungus,Magnaporthe grisea

Author(s) -

K Saitoh,

Tsutomu Arie,

Tohru Teraoka,

Isamu Yamaguchi,

Takashi Kamakura

Publication year - 2003

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.67.651

Subject(s) - magnaporthe grisea , mutant , gene , biology , fungus , phenotype , calcium , magnaporthe , strain (injury) , pathogenicity , genetics , mutation , microbiology and biotechnology , botany , chemistry , anatomy , oryza sativa , organic chemistry

We isolated a neuronal calcium sensor 1/frequenin-like gene, Mg-NCS-1, from Magnaporthe grisea and evaluated the phenotypes of null-mutants of the gene. The putative Mg-NCS-1 protein showed high similarity to the other NCS-1 proteins. The null-mutants had normal growth and pathogenicity similar to the parental strain, but their growth was suppressed in high concentrations of Ca2+ or acidic conditions.

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