Purification and Characterization of Extracellular β-Galactosidase Secreted by Supension Cultured Rice (Oryza sativaL.) Cells | Zendy

Satoshi Kaneko | Zendy; Hideyuki Kobayashi | Zendy

Open Access

Purification and Characterization of Extracellular β-Galactosidase Secreted by Supension Cultured Rice (Oryza sativaL.) Cells

Author(s) -

Satoshi Kaneko,

Hideyuki Kobayashi

Publication year - 2003

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.67.627

Subject(s) - oryza sativa , extracellular , enzyme , galactose , biochemistry , sepharose , chemistry , chromatography , biology , gene

A beta-galactosidase was purified 1300-fold by lactosyl-Sepharose 4B and Sephacryl S-200 column chromatographies from the cultured medium of a rice-cell suspension. The purified enzyme appeared as 47 kD and 40 kD polypeptides on SDS-PAGE and had a specific activity of 65.1 units/mg. Optimum activity was observed at pH 3.5 and 60 degrees C. The enzyme released galactose from galactoxyloglucan and pectic galactans.

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