In vivoVisualization of the Distribution of a Secretory Protein inAspergillus oryzaeHyphae Using the RntA-EGFP Fusion Protein | Zendy

Kumiko Masai | Zendy; Junichi Maruyama | Zendy; Harushi Nakajima | Zendy; Katsuhiko Kitamoto | Zendy

Open Access

In vivoVisualization of the Distribution of a Secretory Protein inAspergillus oryzaeHyphae Using the RntA-EGFP Fusion Protein

Author(s) -

Kumiko Masai,

Junichi Maruyama,

Harushi Nakajima,

Katsuhiko Kitamoto

Publication year - 2003

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.67.455

Subject(s) - green fluorescent protein , aspergillus oryzae , fusion protein , microbiology and biotechnology , secretory pathway , bimolecular fluorescence complementation , heat shock protein , fluorescence microscope , secretory protein , vacuole , secretion , biology , chemistry , fluorescence , yeast , gene , biochemistry , cytoplasm , golgi apparatus , endoplasmic reticulum , recombinant dna , enzyme , physics , quantum mechanics

A fusion gene encoding ribonuclease T1-EGFP (rntA-egfp) was constructed and expressed to use it as a tool for studies on the secretory pathway in Aspergillus oryzae. The successful secretion of the intact RntA-EGFP fusion protein was detected by fluorescence measurement and Western analysis. With use of the RntA-EGFP system, we were able to see high fluorescence at hyphal tips and observe concentrated fluorescence at septa in basal cells during growth at optimal conditions. Cold or heat shock during growth caused the accumulation of EGFP fluorescence in vacuoles.

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