A Transient RNA Interference Assay System UsingArabidopsisProtoplasts | Zendy

Chung-Il An | Zendy; Aki SAWADA | Zendy; Eiichiro Fukusaki | Zendy; Akio Kobayashi | Zendy

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A Transient RNA Interference Assay System UsingArabidopsisProtoplasts

Author(s) -

Chung-Il An,

Aki SAWADA,

Eiichiro Fukusaki,

Akio Kobayashi

Publication year - 2003

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.67.2674

Subject(s) - rna interference , rna silencing , arabidopsis , biology , arabidopsis thaliana , gene silencing , protoplast , microbiology and biotechnology , rna , transgene , gene , functional genomics , mutant , computational biology , genetics , genomics , genome

Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.

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