Production of Functional Lectin inPichia pastorisDirected by Cloned cDNA fromAleuria aurantia | Zendy

Koh Amano | Zendy; Mitsuyo Takase | Zendy; Akikazu Ando | Zendy; Yoshiho Nagata | Zendy

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Production of Functional Lectin inPichia pastorisDirected by Cloned cDNA fromAleuria aurantia

Author(s) -

Koh Amano,

Mitsuyo Takase,

Akikazu Ando,

Yoshiho Nagata

Publication year - 2003

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.67.2277

Subject(s) - pichia pastoris , thermostability , cd69 , lectin , complementary dna , yeast , escherichia coli , plasmid , recombinant dna , c type lectin , glycosylation , pichia , biology , microbiology and biotechnology , biochemistry , gene , enzyme , cytotoxic t cell , il 2 receptor , in vitro

A plasmid bearing a nucleotide sequence of fucose-specific lectin of Aleuria aurantia was constructed and expressed in a methylotrophic yeast, Pichia pastoris. The product showed almost the same hemagglutinating activity as the lectin produced in Escherichia coli, the properties of which were quite similar to the native one. Because of glycosylation of the product, the molecular mass was larger than that of the native one, and it acquired higher thermostability.

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