350-kDa Royal Jelly Glycoprotein (Apisin), Which Stimulates Proliferation of Human Monocytes, Bears the β1-3GalactosylatedN-Glycan: Analysis of theN-Glycosylation Site | Zendy

Mariko Kimura | Zendy; Yoshinobu Kimura | Zendy; Kazunori TSUMURA | Zendy; Kiyoshi Okihara | Zendy; Hiroyuki Sugimoto | Zendy; Hideo Yamada | Zendy; Masami Yonekura | Zendy

Open Access

350-kDa Royal Jelly Glycoprotein (Apisin), Which Stimulates Proliferation of Human Monocytes, Bears the β1-3GalactosylatedN-Glycan: Analysis of theN-Glycosylation Site

Author(s) -

Mariko Kimura,

Yoshinobu Kimura,

Kazunori TSUMURA,

Kiyoshi Okihara,

Hiroyuki Sugimoto,

Hideo Yamada,

Masami Yonekura

Publication year - 2003

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.67.2055

Subject(s) - glycan , glycoprotein , glycosylation , biochemistry , chemistry , microbiology and biotechnology , biology

While doing a structural analysis of minor component N-glycans linked to 350-kDa royal jelly glycoprotein (RJGP), which stimulates the proliferation of human monocytes, we found that a Galbeta1-3GlcNAcbeta1-4Man unit occurs on the insect glycoprotein. The structure of the fluorescence-labeled N-glycan was analyzed by sugar component analysis, IS-MS, and (1)H-NMR. The structural analysis showed that the 350-kDa RJGP bears Galbeta1-3GlcNAcbeta1-4(GlcNAcbeta1-2)Manalpha1-3 (Manalpha1-3Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc, suggesting this insect glycoprotein is one of the substrates for both beta1-3 galactosyl and beta1-4 N-acetylglucosamininyl transferases. To our knowledge, this is the first report that succeeded in identifying an insect glycoprotein bearing the beta1-3 galactosylated N-glycan.

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