Construction of a Homologous Selectable Marker Gene forLentinula edodesTransformation | Zendy

Toshikazu Irie | Zendy; Toshitsugu Sato | Zendy; Kumiko Saito | Zendy; Yoichi Honda | Zendy; Takashi Watanabe | Zendy; Masaaki Kuwahara | Zendy; Hitoshi Enei | Zendy

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Construction of a Homologous Selectable Marker Gene forLentinula edodesTransformation

Author(s) -

Toshikazu Irie,

Toshitsugu Sato,

Kumiko Saito,

Yoichi Honda,

Takashi Watanabe,

Masaaki Kuwahara,

Hitoshi Enei

Publication year - 2003

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.67.2006

Subject(s) - lentinula , selectable marker , transformation (genetics) , transformation efficiency , mutant , gene , mushroom , point mutation , biology , restriction enzyme , genetics , microbiology and biotechnology , chemistry , food science , agrobacterium

We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.

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