Preparation of Antiserum against RatΔ6-Desaturase and Its Use to Evaluate the Desaturase Protein Levels in Rats Treated with Gemfibrozil, a Ligand for Peroxisome Proliferator-… | Zendy

Yôko Shôji | Zendy; Ayako Sanekata | Zendy; Masao Sato | Zendy; Katsumi Imaizumi | Zendy

Open Access

Preparation of Antiserum against RatΔ6-Desaturase and Its Use to Evaluate the Desaturase Protein Levels in Rats Treated with Gemfibrozil, a Ligand for Peroxisome Proliferator-…

Author(s) -

Yôko Shôji,

Ayako Sanekata,

Masao Sato,

Katsumi Imaizumi

Publication year - 2003

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.67.1177

Subject(s) - gemfibrozil , antiserum , stearoyl coa desaturase , peroxisome , microsome , enzyme , chemistry , biochemistry , receptor , biology , gene , gene expression , antibody , immunology , cholesterol

Anti-rat delta6-desaturase serum was produced by immunizing rabbits with the 14 N-terminal amino acids (2-15) of rat delta6-desaturase. The antiserum prevented the enzymatic activity of delta6-desaturase in microsomes. Subsequently, the antiserum was used to demonstrate that gemfibrozil, a ligand for peroxisome proliferator-activated receptor alpha, is involved in activating delta6-desaturase gene expression, thereby elevating the protein level and the activity.

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