Gene Expression during PiDeficiency inPholiota nameko: Accumulation of mRNAs for Two Transporters | Zendy

Yuji Tasaki | Zendy; Yuki Kamiya | Zendy; A. Azwan | Zendy; Takashi Hara | Zendy; Toshio Joh | Zendy

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Gene Expression during P_iDeficiency inPholiota nameko: Accumulation of mRNAs for Two Transporters

Author(s) -

Yuji Tasaki,

Yuki Kamiya,

A. Azwan,

Takashi Hara,

Toshio Joh

Publication year - 2002

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.66.790

Subject(s) - gene , northern blot , complementary dna , microbiology and biotechnology , biology , southern blot , gene expression , transporter , blot , cdna library , genetics

The effects of Pi deficiency on gene expression in Pholiota nameko were examined. A cDNA library was constructed from poly(A)+ RNA isolated from mycelia cultured in Pi-depleted (P-) media, and 150 clones corresponding to Pi deficiency-inducible (pdi) genes were selected by differential hybridization with probes prepared from poly(A)+ RNAs from the mycelia cultured in the Pi-supplied (P+) and P- media. These clones were considered to derive from 31 genes by cross-hybridization. Northern blot analysis showed that these pdi genes were expressed in various patterns during Pi deficiency. Among the clones, the DNA sequences of pdi85 and pdi343 were analyzed. The deduced amino acid sequences indicated that they have structural similarities to Pi and metabolite transporters.

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