Isolation and Characterization ofcbbLandcbbSGenes Encoding Form I Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large and Small Subunits inNitrosomonassp.… | Zendy

Ryuichi Hirota | Zendy; Junichi Kato | Zendy; Hiromu Morita | Zendy; Akio Kuroda | Zendy; Tsukasa Ikeda | Zendy; Noboru Takiguchi | Zendy; Hisao Ohtake | Zendy

Open Access

Isolation and Characterization ofcbbLandcbbSGenes Encoding Form I Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large and Small Subunits inNitrosomonassp.…

Author(s) -

Ryuichi Hirota,

Junichi Kato,

Hiromu Morita,

Akio Kuroda,

Tsukasa Ikeda,

Noboru Takiguchi,

Hisao Ohtake

Publication year - 2002

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.66.632

Subject(s) - ribulose 1,5 bisphosphate , rubisco , nitrobacter , nitrosomonas , ribulose , biochemistry , nitrosomonas europaea , oxygenase , chemistry , biology , escherichia coli , lac operon , gene , nitrification , organic chemistry , nitrogen

The cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large and small subunits in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11 were cloned and sequenced. The deduced gene products, CbbL and CbbS, had 93 and 87% identity with Thiobacillus intermedius CbbL and Nitrobacter winogradskyi CbbS, respectively. Expression of cbbL and cbbS in Escherichia coli led to the detection of RubisCO activity in the presence of 0.1 mM isopropyl-beta-D-thiogalactopyranoside (IPTG). To our knowledge, this is the first paper to report the genes involved in the carbon fixation reaction in chemolithotrophic ammonia-oxidizing bacteria.

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