Identification of an Indispensable Amino Acid for ppGpp Synthesis ofEscherichia coliSpoT Protein | Zendy

Chizuko FUJITA | Zendy; Maki Maeda | Zendy; Takako Fujii | Zendy; Ryoko Iwamoto | Zendy; Kenji Ikehara | Zendy

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Identification of an Indispensable Amino Acid for ppGpp Synthesis ofEscherichia coliSpoT Protein

Author(s) -

Chizuko FUJITA,

Maki Maeda,

Takako Fujii,

Ryoko Iwamoto,

Kenji Ikehara

Publication year - 2002

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.66.2735

Subject(s) - escherichia coli , stringent response , amino acid , biology , biochemistry , chemistry , gene

Amino acid substitutions were introduced into a structurally flexible and highly conserved region of Escherichia coli SpoT protein. SpoT protein changed from Asp to Ala at the 293rd position did not restore cell growth of E. coli CF8295 (delta relA, delta spoT) and did not accumulate ppGpp in the cell, suggesting that the Asp293 is indispensable for ppGpp synthesis of the protein.

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