A New Polypeptide Toxin from the Nematocyst Venom of an Okinawan Sea AnemonePhyllodiscus semoni(Japanese name “unbachi-isoginchaku”) | Zendy

Hiroshi Nagai | Zendy; Naomasa Oshiro | Zendy; Kyoko TakuwaKuroda | Zendy; Setsuko Iwanaga | Zendy; Masatoshi Nozaki | Zendy; Terumi Nakajima | Zendy

Open Access

A New Polypeptide Toxin from the Nematocyst Venom of an Okinawan Sea AnemonePhyllodiscus semoni(Japanese name “unbachi-isoginchaku”)

Author(s) -

Hiroshi Nagai,

Naomasa Oshiro,

Kyoko TakuwaKuroda,

Setsuko Iwanaga,

Masatoshi Nozaki,

Terumi Nakajima

Publication year - 2002

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.66.2621

Subject(s) - sea anemone , venom , nematocyst , toxin , biology , sting , complementary dna , median lethal dose , ed50 , hemolysis , toxicity , microbiology and biotechnology , chemistry , biochemistry , cnidaria , gene , botany , immunology , coral , ecology , in vitro , organic chemistry , engineering , aerospace engineering

The venomous sea anemone Phyllodiscus semoni causes cases of severe stinging. We isolated Phyllodiscus semoni toxin 20A (PsTX-20A), a hemolytic and lethal polypeptide (20 kDa), from the nematocyst venom of this species for the first time. Furthermore, we sequenced the cDNA encoding PsTX-20A. The deduced amino acid sequence of PsTX-20A showed that this toxin was a new member of the actinoporin family, which consists of several cytolytic polypeptides originating from sea anemones. PsTX-20A showed lethal toxicity to the shrimp Palaemon paucidens when administered via intraperitoneal injection (LD50, 50 microg/kg) and hemolytic activity toward 0.8% sheep red blood cells (ED50, 80 ng/ml).

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