Potentiation of Nerve Growth Factor-Induced Neurite Outgrowth in PC12 Cells by a Coptidis Rhizoma Extract and Protoberberine Alkaloids | Zendy

K. Shigeta | Zendy; Keisuke Ootaki | Zendy; Hideki Tatemoto | Zendy; Tsutomu Nakanishi | Zendy; Akira Inada | Zendy; Norio Muto | Zendy

Open Access

Potentiation of Nerve Growth Factor-Induced Neurite Outgrowth in PC12 Cells by a Coptidis Rhizoma Extract and Protoberberine Alkaloids

Author(s) -

K. Shigeta,

Keisuke Ootaki,

Hideki Tatemoto,

Tsutomu Nakanishi,

Akira Inada,

Norio Muto

Publication year - 2002

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.66.2491

Subject(s) - berberine , neurite , palmatine , nerve growth factor , chemistry , long term potentiation , coptisine , pharmacology , cytotoxicity , alkaloid , acetylcholinesterase , in vitro , biochemistry , biology , stereochemistry , enzyme , receptor

A methanol extract of Coptidis Rhizoma effectively enhanced the outgrowth of neurite in PC12 cells induced by nerve growth factor (NGF). Following solvent partition and preparative HPLC, berberine was isolated as the major active compound. Berberine enhanced the proportion of neurite-bearing cells in a dose-dependent manner without cytotoxicity. Its structural relatives, palmatine and coptisine, showed a slightly weaker NGF-enhancing effect than berberine. These three alkaloids inhibited acetylcholinesterase activity at a level comparable to that of physostigmine, but this inhibition was not responsible for the potentiation of NGF-induced neurite outgrowth. It is demonstrated for the first time that protoberberine alkaloids potentiated the NGF-induced differentiation of neural cells.

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