Identification of a Wheat Allergen, Tri a Bd 36K, as a Peroxidase
Author(s) -
Hiromi Yamashita,
Yoko NANBA,
Miki ONISHI,
Masumi Kimoto,
Miki HIEMORI,
Hideaki Tsuji
Publication year - 2002
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.66.2487
Subject(s) - peroxidase , allergen , glycan , chemistry , biochemistry , immunoglobulin e , enzyme , antibody , microbiology and biotechnology , biology , glycoprotein , immunology , allergy
A 36-kDa allergen, Tri a Bd 36K, was purified from wheat albumin and characterized. The protein was similar to barley peroxidase BP-1 both in its amino acid sequence and peroxidase activity. The enzyme seemed to contain L-fucose and D-mannose and the glycan moiety reacted with IgE antibodies in a patient's serum.
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