Cloning and Expression of the Exo-β-D-1,3-glucanase Gene (exgS) fromAspergillus saitoi | Zendy

Ken Oda | Zendy; Shin Kasahara | Zendy; Youhei Yamagata | Zendy; Keietsu Abe | Zendy; Tasuku Nakajima | Zendy

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Cloning and Expression of the Exo-β-D-1,3-glucanase Gene (exgS) fromAspergillus saitoi

Author(s) -

Ken Oda,

Shin Kasahara,

Youhei Yamagata,

Keietsu Abe,

Tasuku Nakajima

Publication year - 2002

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.66.1587

Subject(s) - glucanase , aspergillus oryzae , recombinant dna , amino acid , biology , gene , intron , cloning (programming) , heterologous expression , peptide sequence , genomic dna , open reading frame , genetics , biochemistry , enzyme , computer science , programming language

A gene of exo-1,3-beta-D-glucanase (exgS) was cloned from a koji mold, Aspergillus saitoi, genomic DNA using PCR. The exgS has an ORF comprising 2832 bp, which contains one intron of 45 bp, and encodes 945 amino acids. The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an Aspergillus oryzae strain that carried an expression vector containing full length of the exgS. The N-terminal amino acid sequences of the recombinant exo-1,3-beta-D-glucanase (ExgS) were identical to that of native ExgS from A. saitoi.

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