An Enzymatic Fluorometric Assay for Pyridoxal with High Specificity and Sensitivity | Zendy

Yanee Trongpanich | Zendy; Mitsunori Mito | Zendy; Toshiharu Yagi | Zendy

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An Enzymatic Fluorometric Assay for Pyridoxal with High Specificity and Sensitivity

Author(s) -

Yanee Trongpanich,

Mitsunori Mito,

Toshiharu Yagi

Publication year - 2002

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.66.1152

Subject(s) - pyridoxal , pyridoxal 5 phosphate , chemistry , chromatography , calibration curve , enzyme , detection limit , pyridoxal phosphate , biochemistry , cofactor

An enzymatic fluorometric assay for pyridoxal with pyridoxal dehydrogenase was developed. The detection limit was about 10 pmol: the calibration curve of pyridoxal showed high linearity (r=0.993). The values obtained by this method correlated well with those by the HPLC method. The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation of pyridoxal 5'-phosphate by column chromatography.

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