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2,3-Diketo-L-gulonic acid (DKG) is an important intermediate product of oxidative degradation of L-ascorbic acid (AsA) in both biological and food systems, but the physiological function of DKG is still unclear. In this study, it was found that DKG had a strong antioxidative effect on copper-dependent oxidative modification of yolk lipoprotein (YLP), on the basis of both the decreased electrophoretic mobility and longer lag time of conjugated diene formation in a concentration-dependent manner. DKG is known to be very unstable and easily converts into two delta-lactones of DKG, the 3,4-enediol form of DKG delta-lactone (3,4-DKGL) and 2,3-enediol form of DKG delta-lactone (2,3-DKGL) depending on both pH and temperature. 3,4-DKGL was thought to be the first degradation product of DKG and could play an antioxidative role in the oxidation of lipoproteins induced by copper ion or peroxyl radicals in neutral aqueous solution.

Effects of 2,3-Diketo-L-gulonic Acid on the Oxidation of Yolk Lipoprotein