Green Marker for Colonies of Bacillus subtilis | Zendy

Mitsuhiro Itaya | Zendy; Syed Shaheduzzaman | Zendy; Kuniko Matsui | Zendy; Akira Omori | Zendy; Takashi Tsuji | Zendy

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Green Marker for Colonies of Bacillus subtilis

Author(s) -

Mitsuhiro Itaya,

Syed Shaheduzzaman,

Kuniko Matsui,

Akira Omori,

Takashi Tsuji

Publication year - 2001

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.65.579

Subject(s) - bacillus subtilis , plasmid , green fluorescent protein , gene , biology , translation (biology) , bacillaceae , microbiology and biotechnology , fluorescence , bacteria , genetics , messenger rna , physics , quantum mechanics

A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.

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