Factors in Bovine Colostrum that enhance the Migration of Human Fibroblasts in Type I Collagen Gels | Zendy

Yoshiharu Takayama | Zendy; Katsuhiro Kitsunai | Zendy; Koko Mizumachi | Zendy

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Factors in Bovine Colostrum that enhance the Migration of Human Fibroblasts in Type I Collagen Gels

Author(s) -

Yoshiharu Takayama,

Katsuhiro Kitsunai,

Koko Mizumachi

Publication year - 2001

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.65.2776

Subject(s) - size exclusion chromatography , colostrum , chemistry , sodium , motility , elution , chromatography , ion chromatography , ion exchange , biochemistry , ion , biology , immunology , antibody , microbiology and biotechnology , enzyme , organic chemistry

Bovine colostrum has an activity that increases the migration of WI38 fibroblasts. We evaluated the motility of fibroblasts by their ability to contract collagen gels. Part of the activity was absorbed by anion-exchange chromatography at pH 6.4, and eluted by 0.2-0.3 M sodium chloride. The activity was separated into many fractions corresponding to 20-150 kDa by gel filtration chromatography under acidic conditions. The major peak of the activity coincided with 50-70 kDa.

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