Sandwich Enzyme-linked Immunosorbent Assay System for Micro-detection of the Wheat Allergen, Tri a Bd 17 K | Zendy

Hiromi Yamashita | Zendy; Masumi Kimoto | Zendy; Miki HIEMORI | Zendy; Misako Okita | Zendy; Kazuhiko Suzuki | Zendy; Hideaki Tsuji | Zendy

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Sandwich Enzyme-linked Immunosorbent Assay System for Micro-detection of the Wheat Allergen, Tri a Bd 17 K

Author(s) -

Hiromi Yamashita,

Masumi Kimoto,

Miki HIEMORI,

Misako Okita,

Kazuhiko Suzuki,

Hideaki Tsuji

Publication year - 2001

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.65.2730

Subject(s) - allergen , monoclonal antibody , epitope , chemistry , microbiology and biotechnology , antibody , peptide , enzyme , chromatography , immunology , allergy , medicine , biology , biochemistry

Seven monoclonal antibodies (mAbs) against the wheat allergen, Tri a Bd 17 K, were prepared to obtain mAbs suitable for a sandwich enzyme-linked immunosorbent assay (sandwich ELISA) for determination of the allergen. Two of the mAbs strongly immunoblotted the allergen purified from wheat flour. However, only one (1G11) of them was found to be suitable for sandwich ELISA. Epitope mapping against mAb-1G11 on the allergen showed that the mAb recognized the peptide containing Lys-38 and Gln-39 of the allergen. We developed a sandwich ELISA method consisting of Aleuria aurantia lectin for fixing the allergen and 1G11 as the first antibody that enabled 4-4,000 ng/well of the allergen to be determined.

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