Molecular Characterization of β-tubulin gene from Pleurotus sajor-caju
Author(s) -
BeomGi Kim,
YoungBok Yoo,
SukTae Kwon,
Yumi Magae
Publication year - 2001
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.65.2280
Subject(s) - intron , gene , tubulin , pleurotus , genetics , biology , coding region , microtubule , botany , mushroom
A beta-tubulin gene (TUB1) from the basidiomycete Pleurotus sajor-caju was sequenced. TUB1 encodes a 446-amino-acid protein. The coding region is interrupted by 9 introns, all of which had a 5'-GTRNGT... YAG-3' sequence at the boundaries. Locations of the introns in TUB1 were common between the beta-tubulin genes of other basidiomycetes, but not with animals, ascomycetes, or plants. This suggests that the introns were inserted independently into the beta-tubulin gene after these divisions had diverged.
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