A Cryptic Plasmid, pA01, from a Compost Bacterium, Bacillus sp. | Zendy

Shuichi Karita | Zendy; Akihito Ohtaki | Zendy; Mioborikawa | Zendy; Kiyohiko Nakasaki | Zendy

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A Cryptic Plasmid, pA01, from a Compost Bacterium, Bacillus sp.

Author(s) -

Shuichi Karita,

Akihito Ohtaki,

Mioborikawa,

Kiyohiko Nakasaki

Publication year - 2001

Publication title -

bioscience biotechnology and biochemistry

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.65.226

Subject(s) - biology , orfs , plasmid , genetics , insertion sequence , open reading frame , nucleic acid sequence , bacillus coagulans , 16s ribosomal rna , sequence analysis , bacteria , microbiology and biotechnology , peptide sequence , gene , transposable element , genome

The complete nucleotide sequence of a new cryptic plasmid, pAO1 isolated from a compost bacterium Bacillus sp., has been analyzed. Analysis of the PCR-based 16S rRNA sequence showed the bacterium harboring pAO1 was closely related to Bacillus pallidus. The plasmid pAO1 was 3,325 bp in size. Two open reading frames, ORF1 and ORF2, encoding putative polypeptides of 248 and 290 amino acids, respectively, were identified within the sequence. The ORF1 has a limited sequence similarity to an integrase/recombinase, while the ORF2 has high similarity with the replication protein of pBC1 from Bacillus coagulans. A putative origin sequence for a plus-strand was located between ORFs. Southern blot analysis indicates this plasmid replicates via a rolling circle-type mechanism.

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