Sequence Analysis and Overexpression of a Pectin Lyase Gene (pel1) from Aspergillus oryzae KBN616 | Zendy

Noriyuki KITAMOTO | Zendy; Shoko Yoshino-Yasuda | Zendy; Kunio Ohmiya | Zendy; Norihiro Tsukagoshi | Zendy

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Sequence Analysis and Overexpression of a Pectin Lyase Gene (pel1) from Aspergillus oryzae KBN616

Author(s) -

Noriyuki KITAMOTO,

Shoko Yoshino-Yasuda,

Kunio Ohmiya,

Norihiro Tsukagoshi

Publication year - 2001

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.65.209

Subject(s) - aspergillus oryzae , gene , biology , peptide sequence , nucleic acid sequence , aspergillus niger , amino acid , biochemistry , pectin , signal peptide , microbiology and biotechnology , enzyme

A gene (pel1) encoding pectin lyase (Pel1) was isolated from a shoyu koji mold, Aspergillus oryzae KBN616, and characterized. The structural gene comprised 1,196 bp with a single intron. The ORF encoded 381 amino acids with a signal peptide of 20 amino acids. The deduced amino acid sequence showed high similarity to those of Aspergillus niger pectin lyases and Glomerella cingulata PnlA. The pel1 gene was successfully overexpressed under the promoter of the A. oryzae TEF1 gene. The molecular mass of the recombinant pectin lyase substantially coincided with that calculated based on nucleotide sequence.

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