Enzyme-assisted Preparation of D-tert.-Leucine | Zendy

Kurt Laumen | Zendy; Oreste Ghisalba | Zendy; Kurt Auer | Zendy

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Enzyme-assisted Preparation of D-tert.-Leucine

Author(s) -

Kurt Laumen,

Oreste Ghisalba,

Kurt Auer

Publication year - 2001

Publication title -

bioscience biotechnology and biochemistry

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.65.1977

Subject(s) - saponification , bacillus licheniformis , hydrolysis , chemistry , leucine , enzyme , protease , monomer , ethyl ester , organic chemistry , chromatography , biochemistry , amino acid , bacteria , bacillus subtilis , polymer , biology , genetics

Optically pure D-tert.-leucine was obtained by the enzymatic hydrolysis of (+/-)-N-acetyl-tert. leucine chloroethyl ester after about 50% conversion, this being catalyzed by a protease from Bacillus licheniformis (Alcalase), and subsequent acidic saponification of the recovered ester. Among the methyl, ethyl, octyl, chloroethyl and trichloroethyl esters, the chloroethyl ester exhibited the highest rate of hydrolysis.

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